Effect of protein kinase C signal pathway on resistin expression in 3T3-L1 adipocytes / 中国病理生理杂志

ABSTRACT

AIM: To investigate the effect of protein kinase C on resistin expression in 3T3-L1 adipocytes.METHODS: The differentiated 3T3-L1 adipocytes were incubated with 50 nmol/L phorbol 12-myristate 13-acetate(PMA) or(5 ?mol/L) Ro-31-8220 for 24 h.Expression of resistin mRNA was detected by RT-PCR and expression of resistin protein was detected by Western blotting.RESULTS: Compared with control,PMA increased the expression of resistin mRNA and protein in 3T3-L1 adipocytes significantly(P