Human bone marrow mesenchymal stem cells differentiate toward endothelial lineagein vitro / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-528388
ABSTRACT
AIM:
To investigate the cytological basis and differentiating conditions of human bone marrowmes-enchymal stem cells(hMSCs) differentiated into cells of the endothelial lineagein vitro.METHODS:
hMSCs were isolatedby density gradient centrifugation and fractionated on a 1 073 g/L Percoll.The combination of VEGF165and various matrixproteins including fibronectin(FN) and typeⅠ collagen(Col) was used to induce hMSCsin vitro.Cells were character-ized by immunohistochemistry,cytochemistry,FACS and ultrastructure to identify and detect the differentiated populationand markers.RESULTS:
hMSCs was positive for KDR.PAS reaction was positive and ultrastructure of hMSCs showedglycogen-pool in ectoplasm.Glycogen reducing or disappear suggested that stem cells have occurred differentiation.In-duction of hMSCs resulted in the increase of KDR,?1integrin and CD34.CONCLUSION:
hMSCs were induced to atransit population(TP) that differentiated toward the endothelial progenitor cells(EPC),but not a really EPC.hMSCspedigree diagram of differentiation was hMSCs→TP→EPC→endothelial cells(ECs).
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2000
Type:
Article
Similar
MEDLINE
...
LILACS
LIS