Extraction and Purification of Omeprazole Metabolites in Human Urine / 中国药房
China Pharmacy
; (12)2005.
Article
in Zh
| WPRIM
| ID: wpr-528584
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:To presume the structures and determine the contents of two metabolites of omeprazole in human urine after extraction and purification.METHODS:24 healthy volunteers were assigned to receive single oral dose of 40mg omepazole capsules,whose urinary samples collected within 12 hours after administration were extracted and concentrated with diethyl ether,and separated by HPLC.The relative purified metabolites were detected by mass spectrum,the structures of which were presumed and the contents were computed.RESULTS: The isolated 2 metabolites of omeprazole were presumed to be pyridine 5'— or 3'— methyl oxidation-generated hydroxy sulfone metabolite and 5'—methy hydroxylation thioether metabolite in pyridine ring,and the contents of which were 96.54% and 97.26%,respectively.CONCLUSION:The metabolites of omeprazole isolated from urinary samples by the method mentioned above were of high purity.
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
China Pharmacy
Year:
2005
Type:
Article