Effect of curcumin on the proliferation and apoptosis in LNCap cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-528662
ABSTRACT
AIM:
To observe the effect of curcumin on the proliferation and apoptosis of prostate cancer cell line LNCap.METHODS:
LNCap cells were treated with different doses(10 ?mol/L,20 ?mol/L,30 ?mol/L,40 ?mol/L) of curcumin and its effects were analyzed in cell growth and apoptosis by microscope,MTT colorimetric assay and flow cytometry.The expression of prostate specific antigen(PSA) was measured by AXSYM~(TM) system-chemical luciferase methods and expression of androgen receptor (AR) was detected by Western blotting.RESULTS:
The results showed that curcumin inhibited the proliferation of LNCap cells.The cell growth was inhibited by curcumin in a dose dependent manner and the optimal dose and time was 40 ?mol/L,24 h.Curcumin induced apoptosis in LNCap cells,the most dramatic dose was 40(?mol/L) curcumin,at this dose the apoptosis rate was 9.23%. Curcumin inhibited the expression of PSA in LNCap cells and the most dramatic dose and time was 40 ?mol/L,24 h.The PSA in this group was 20% of the control group.Curcumin inhibited the expression of AR on prostate cancer cells.CONCLUSION:
Curcumin decreases proliferation and induces apoptosis in LNCap cells in a dose-dependent manner.Curcumin also inhibites the expression of PSA and AR on LNCap cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2000
Type:
Article
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