Effects of peroxisome proliferators-activatied receptor gamma on the biological characteristics of hepatic stellate cells / 中国普通外科杂志

ABSTRACT

Objective To study the effects of peroxisome proliferators-activated receptor gamma (PPA?r) on the biological characteristics of hepatic stellate cells (HSCs); Methods The activated HSCs were divided into four groups control group, rosiglitazone group, GW9662 + rosiglitazone group and GW9662 group. The cell proliferation was determined with MTT colorimetric assay, The cell apoptosis was studied with flow cytometry. The expression of PPA?r, Type I collagen were detected by RT-PCR, Western blot and immunocytochemistry. Results MTT of HSCs in rosiglitazone group was (0. 49?0. 06) significantly lower than in control group ( 1. 00?0. 045 ), GW9662 group (0. 89?0. 043 ) , GW9662 + rosiglitazone group (0.78?0.049)(t = 15.59,14.68,8.07, P