Comparative Study on the Antineoplastic Effect of Non-alkaloid Components from Gelsemium Elegans Benth in Vitro and in Vivo / 中国药房

ABSTRACT

OBJECTIVE:To evaluate the antineoplastic effects of non-alkaloid components from Gelsemium elegans Benth in vitro and in vivo and to study the correlation between antineoplastic effects and toxicity.METHODS:The cell proliferation inhibition effect of every component on tumor cells in vitro was studied by MTT method,the antineoplastic activity of non-alkaloid components were comprehensively evaluated through acute toxicity testing on mice,the inhibition effect test on H 22 of liver tumor-bearing mice and the test on the effect on immunizing index.RESULTS:The antineoplastic activity of non-alkaloid in Gelsemium elegans in vitro surpassed alkaloid,and in which the component 2 could significantly inhibit H 22 tumor growth in mice,the thymus gland indexes were significantly higher in treatment group than non-treatment tumor-bearing mice.However,the other components showed no significant effect on tumor growth and those with stronger toxicity showed no antineoplastic activity.CONCLUSIONS:The non-alkaloid components from Gelsemium elegans Benth show anti-neoplastic effects and enhancement property on immune function,however,its antineoplastic effects in vitro and in vivo have no obvious correlation with toxicity.