A Comparative Morphology Study on the Growth of Cells Isolated from Different Regions of Neonatal Rat's CNS / 昆明医科大学学报

ABSTRACT

Objective To isolate and identify the cells from the cerebral cortex,cerebellar cortex,hippocampus,sub-ventricle region,brain stem and the spinal cord of neonatal rats and observe their growth morphology in vitro.Methods The cells were incubated from different regions of the CNS of neonatal rats by using DMEM/F12 media added with 10% bovine serum,and their growth morphology was observed by using inverted phase contrast microscope;then in the 10th day after incubation cells were fixed and immunocytochemical method was used to detect specific NSE antigen of the neurons and specific GFAP antigen of the astrocytes.Results Both neurons and astrocytes were studied in each region and they bloomed in the 10th day after incubation.Neurons had big triangular or ellipsoidal cell bodies surrounded with a halo and had robust nervous process which interlaced each other around cell bodies.The astrocytes had an ellipsoidal nucleus located at one side of the cell body and they had abundant processes branching profusely.Conclusion A method of culturing cells from different regions of the CNS of neonatal rats was described.A comparative morphology study of their growth was made and neurons and astrocytes of all the regions studied were identified.