Immune function of dendritic cells in patients with hepatocellular carcinoma / 中国病理生理杂志

ABSTRACT

AIM:To investigate the immune function of dendritic cells(DCs)in patients with hepatocellular carcinoma(HCC).METHODS:The DCs were cultured from human peripheral blood mononuclear cells(PBMC)by using GM-CSF and IL-4 for 7 days.Surface molecules CD86,HLA-DR of DCs were detected by flow cytometry.IL-12 production by DCs and IFN-? production by T cells was measured with ELISA and ELISPOT,respectively.Allogenic mixed lymphocyte reaction was detected by MTT assay.RESULTS:CD86 expression in DCs in HCC patients were markedly lower than that in health control(91.7% vs 83.5%,P0.05].However,after stimulated DCs with LPS,IL-12 production in HCC patients was significantly lower than that in health control [(478.6?142.7)ng/L vs(630.0?151.9)ng/L,P