Determination of the Related Substances in Compound Vitamine Injection by RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE:To determine the related compounds in compound vitmine injection by RP-HPLC.METHODS:The determination was performed on C18 column,and the mobile phase was composed of methanol-alcohol(80∶ 20)with a flow rate of 0.5mL? min-1 and detection wavelength of 270nm.RESULTS:The related substances in compound vitamine injection were separated effectively.The total peak area of all foreign materials was no more than 3% of the main peak area of contrast solution,and the peak area of single foreign material was no more than 1.5% of the main peak area of the contrast solution.CONCLUSION:The method is simple and rapid,suitable for the quality control of compounds of compound vitmine injection.