Changes of vascular structure and function after adventitia removal in spontaneously hypertensive rats / 中国病理生理杂志

ABSTRACT

AIM:To study the effect of adventitia removal on vascular structure and function in spontaneously hypertensive rats(SHR).METHODS: Thirteen-week-old male SHR(n=8) were removed their right carotid artery adventitia(SHR control group),8 age-matched male Wistar-Kyoto rats were selected as normal control group(WKY group).With all rats,the right carotid artery adventitia was removed by mechanical and chemical means,and the left carotid artery adventitia was conducted with fake operation for control.After 4 weeks,the velocity of carotid artery was detected by electromagnetic rheometer.Plasma and carotid artery angiotensin Ⅱ(AngⅡ) levels were measured by radioimmunoassay.Lumen cross section area(LA),intraelastic layer area(IELA) and extraelastic layer area(EELA) were measured by computed video processing.The ratio of(IELA-LA)/(EELA-IELA) was calculated.The expression of angiotensin converting enzyme(ACE2),extracellular signal-regulated kinase 1/2(ERK1/2) and protein kinase C-?(PKC-?) protein were determined by immunohistochemistry method.The expression of ACE2 mRNA was evaluated by reverse transcription-polymerase chain reaction(RT-PCR).RESULTS:(1) The hyperplasia of intima in adventitia removal was more significantly exacerbated than that in adventitia integrity(P