Proteomic study on skeletal muscle mitochondrial proteins of chronic hypoxic Wistar rats / 中国病理生理杂志

ABSTRACT

AIM: To explore hypoxia-related mitochondrial proteins in Wistar rats exposed to chronic hypobaric hypoxia.METHODS: Adult male Wistar rats were randomized into chronic hypoxia (30 d) group and control group. Bilateral gastrocnemius were collected and mitochondria were extracted and purified for two dimensional electrophoresis (2-DE) and matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) analysis.RESULTS: Compared to the control, 35 protein spots in chronic hypoxia group were up-regulated (8 protein spots) or down-regulated (27 protein spots), 8 of which were identified as F1-ATPase alpha subunit, voltage-dependent anion channel 1, androgen regulated protein, tripartite motif protein 50, hydroxyacyl-coenzyme A dehydrogenase alpha subunit and mitochondrial F1 complex gamma subunit.CONCLUSION: Our results suggest that hypoxia exposure may affect mitochondrial electron transport chain, oxidative phosphorylation and lipid metabolism.