Determination of Plasma Diclofenac Sodium by SPE-HPLC / 中国药房
China Pharmacy
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-529025
ABSTRACT
OBJECTIVE:
To establish an SPE(solid phase extraction)-HPLC method for the determination of diclofenac sodium in human plasma.METHODS:
The separation of diclofenac sodium was performed on ODS-2 column with a detection wavelength of 280nm.The mobile phase was composed of phosphate buffer(pH6.5)-methanol(40∶ 60) at a flow rate of 1.0mL? min-1.RESULTS:
The linear range of diclofenac sodium was 16.2~ 2 024.0ng? mL-1(r=0.998 9).The methodological recovery ranged between 90% to 110% and the extraction recovery was above 90%.Both intraday RSD and inter-day RSD were less than 5%.CONCLUSION:
The method is simple,accurate and reliable,and suitable for the pharmacokinetic study of diclofenac sodium.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2001
Type:
Article
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