Discrepancy of microRNA in different radioresistant nasopharyngeal carcinoma cells / 中国病理生理杂志

ABSTRACT

AIM: To discuss the discrepancy of microRNA (miRNA) in nasopharyngeal carcinoma (NPC) cells CNE-1 and CNE-2 on the basis of validating their different radioresistance.METHODS: Following the effect of X ray on the clones of CNE-1 and CNE-2 cells, the dose-survival curve and biological characteristics of CNE-1 and CNE-2 cells were determined by SigmaPlot software and the linear quadratic model of survival curves analysis. MicroRNAs were detected by Paraflo microfluidic microRNA chip, hybridization images collected using a laser scanner and normalizing the signals using a LOWESS filter. The relationship between the discrepancy of NPC radiosensitivity and the expression of microRNA was predicted according to Targetscan3.1 database (http:www.targetscan.org) after analyzing the data.RESULTS: Compared to CNE-2 cells, 20 microRNAs were gain-of-function and 13 microRNAs loss-of-function in CNE-1 cells among 326 detected microRNAs. 4 miRNAs that one detective value was more than 2000 and 3 folds than the other were hsa-miR-152, hsa-miR-7, hsa-miR-205 and hsa-miR-572. Data showed that radio-sensitivity was relative to the distinct discrepancy of miRNAs. CONCLUSION: The discrepancy of miRNAs is presents in different radioresistant NPC cell lines and related to radiosensitivity.