Determination of Cefuroxime in Experimental Dogs by HPLC / 中国药房
China Pharmacy
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-529455
ABSTRACT
OBJECTIVE:
To establish a HPLC method for the determination of plasma level of cefuroxime in experimental dogs.METHODS:
1. 0mL plasma samples were taken from experimental dogs at different time after intravenous injection of cefuroxime sodium 50mg? kg-1. Following pretreatment, the samples were subjected to determination on XDB-C18 chromatographic column. The mobile phase consisted of CH3CN-1‰ ( NH4) 2SO4 ( 12∶ 88) with a flow rate of 1. 0mL? min-1. The detection wavelength was 273nm and the column temperature was 25℃ . The quantification was performed by external standard method.RESULTS:
Good linear relationship was achieved when the detection concentration of cefuroxime was within the range of 0. 5~ 250? g? ml-1( r=0. 999 5) . The average recovery of cefuroxime was 97. 76~ 116. 00% ( RSD
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2007
Type:
Article
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