Effects of Ruzengxiao on Rats with Hyperplasia of Mammary Glands / 中国药房

ABSTRACT

OBJECTIVE:To observe the efficacy of Ruzengxiao in the prevention and cure of hyperplasia of mammary glands in rats.METHODS:To investigate the therapeutic effect of drugs,the spayed rats were injected i.m with benzoic acid E2 0.8mg? kg-1 qd alt for 90 consecutive days,during the period between 20 and 30 days after modeling,these rats were injected with progesterone 4mg? kg-1 qd alt for 5 consecutive times;30d after modeling,the model animals with hyperplasia of mammary glands were randomized to groups to be treated intragastrically with 0.05% CMC-Na,Ruzengxiao or Tomoxi fen qd for 60d consecutively.Samples were taken at 24h after the last time of drug administration for determination of indexes.RESULTS:After treating the model animals for 2months,the following experimental results were obtainedfor the spayed animals,after injection with estradiol(E2)for 3mo,their index of thymus decreased,indexes of uterus and adrenal glands increased significantly;levels of estrogen receptor(ER)in mammary tissues and serum E2 increased significantly,while the serum progesterone(P)deceased significantly,ratio of E2/testosterone(T)increased significantly;the numbers of lobules of mammary gland increased,hyperplasia of interstitial tissues appeared,secretion of acini increased,and the acini were highly distended,level of prolactin(PRL)increased,while levels of luteinizing hormone(LH)and follicle stimulating hormone(FSH)decreased,but no statistic difference was noted as compared with castration group(spayed animal without administration with E2 and P etc.);the E2-induced increase in indexes of uterus and adrenal gland was significantly inhibited after treatment with Tamoxifen or Ruzengxiao 20g? kg-1 for 2mo;Both Ruzengxiao(at different dosage)and Tomoxifen could significantly increase serum Testosterone level but decrease ratio of E2/T in rats with hyperplasia of mammary glands,showing significant differences as compared with model group.All drugs showed no obvious effect on serum levels of LH,FSH,PRL,P and E2 etc in rats with hyperplasia of mammary glands.CONCLUSION:The above results reveal that Ruzengxiao has satisfactory preventive and curative effects on model rats with hyperplasia of mammary glands.This mechanism might be related to the reduction of serum levels of E2,P and PRL,and the ratio of E2/T.