Determination of Vitamin D_2, Vitamin A and Vitamin E in Cernevit-12 for Injection by HPLC / 中国药房
China Pharmacy
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-529582
ABSTRACT
OBJECTIVE:
To establish a HPLC method for the simultaneously determination of Vitamin D2, Vitamin A and Vitamin E in Cernevit-12 for injection.METHODS:
An Inertsil(ODS-2) C18 column was selected as separation column at 30℃. The mobile phase consisted of acetonitrile-methanol-methylene chloride (80∶10∶10) at a flow rate of 1.0mL?min-1. The detection wavelength was set at 265nm and the injection volume was 10?L.RESULTS:
The linear ranges of Vitamin D2, Vitamin E acetic ester and Vitamin A palmitate were 0.61~1.43?g?mL-1, 222.6~519.4?g?mL-1 and 1.21~2.82mg?mL-1 respectively. The average recoveries were 99.79%, 99.53% and 101.63%, with RSD of 1.66%, 0.184 6%, 0.113 4%.CONCLUSION:
The method is simple, rapid and accurate, and suitable for the determination of Cernevit-12.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2007
Type:
Article
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