The role of cytochrome C in the apoptosis of hepatoma cell induced by flurouracil / 中国普通外科杂志

ABSTRACT

Objective To investigate the role of cytochrome C in the apoptosis of hepatoma cell induced by flurouracil,and to analyze the distribution of cytochrome C in cytosol and the relationship between the cytochrome C distribution and Caspase-9 activation.Methods The human hepatoma HepG2 cells were treated with flurouracil at 1?10-2mol/L and 2,4,8,16,24h respectively.The chang of cytochrome C in HepG2 apoptosis was detected by using Fluorescent Assay Kit;and proteolytic cleavage of caspase-9 and distribution of cytochrome C in cytosol or in mitochondria was analyzed by Western blot.Results Four hours after cells exposure to flurouracil,the caspase-9 activity in HepG2 cells increased gradually and reached the peak at 16h,compared with the control groups,the difference was significant(P