Determination of 3 Kinds of Components in Bikeling Nasal Spray by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a HPLC method for the simultaneously determination of three components in Bikeling nasal spray. METHODS: The column we used was Hypersil ODS and the mobile phase was 0.1%mtriethylamine(adjust pH3.2 with phosphate acid)-acetonitrile (75∶25). The detective wavelength was 256nm and the sample size was 10?L. RESULTS: The linear rangs of ofloxacin, ephedrine hydrochloride and dexamethasone sodium phosphate were 32～320?g?mL-1(r=0.999 9),64～640?g?mL-1(r=1.000 0) and 4～40?g?mL-1(r=1.000 0)respectively, and the average recoveries were 100.05%(RSD=0.32%), 100.17%(RSD=0.48%) and 99.85%(RSD=1.67%),respectively. CONCLUSION: The method was simple and accurate and can be used for the quality control of Bikeling nasal spray