Quantitative Determination of Gastrodin in Tianmujiangya Tablets by RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish an RP-HPLC method for the quantitative determination of Gastrodin in Tianmu-jiangya tablets.METHODS:The chromatographic separation was performed on Shimadzu-C18(150mm?4.6mm, 5?m) column with a column temperature at 40℃. The mobile phase consisted of acetonitrile-water(0.5∶99.5) at a flow rate of 1.0mL?min-1. The detection wavelength was set at 221nm. RESULTS:The linear correlation of Gastrodin was achieved in the range of 0.271 2?g～1.356 0?g(r=0.999 9). The average recovery rate was 100.13%(RSD=1.89%,n=6). CONCLUSION:The method is simple, accurate and specific and with high recovery rate, and it can be used for the quality control of Tianmujiangya tablets.