Effect of Qingluotongbi Granules on Bone Destruction in Rats with Adjuvant-induced Arthritis(AIA) / 中国药房

ABSTRACT

OBJECTIVE:To study the effect of Qinglutongbi granules on bone destruction in rats with adjuvant-induced arthritis (AIA). METHODS:AIA model was established in rats. Qinglutongbi granules were given to the model rats on day 7 for a total of 49 days. Roentgenography of joints of rats was performed for the observation of bone destruction. Synovial cells were taken from each group for cultivation of supernatant liquid, which was then added to cartilage cells that cultured in vitro. The outcome measures were proliferative response of cartilage cells, NO content and expression of antileptic type iNOS in supernatant of cartilage cells. RESULTS:Qinglutongbi granules (high, middle and low dosages) could improve the reduced bone density of joints in hind limb, widened articular cavity space and disorganization of joints in rats. Supernatant liquid that cultivated with synovial cells from AIA rats receiving Qinglutongbi granules at dosages of 7.2 g?kg-1 and 14.4 g?kg-1 could enhance the reproductive activity of chondrocytes and reduce iNOS expression as well as NO content. CONCLUSION:At a certain dosage, Qinglutongbi granules can inhibit the destruction of cartilage and bone in rats.