Topiramate Promotes Proliferation of Neural Stem Cells in Vitro / 中国药房
China Pharmacy
;
(12)1991.
Article
in Chinese
| WPRIM
| ID: wpr-529871
ABSTRACT
OBJECTIVE:
To study the effects of topirmate on proliferation of neural stem cells in vitro.METHODS:
Primary neural stem cells from newborn mice were cultured.The neural stem cells were incubated together with topiramate at 0.4,2 or 10 ?mol?L-1.A vehicle control and positive control were set up,respectively.The quantity and viability of neural stem cells newly generated were analyzed by a convert phase microscope,the neurosphere numbers were counted,and neural stem cells viability was determined and a MTT assay.RESULTS:
The number of neurospheres of groups treated with topiramate at 2 and 10 ?mol?L-1 are 20.67?4.04,49.68?3.79,respectively,and the value of MTT assay of the two groups are 0.354 6?0.070 5,0.501 8?0.036 9,it was higher than the vehicle control neural stem cells(7.34?2.31、0.288 0?0.009 3,P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
1991
Type:
Article
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