Content Determination of Cholesterol Esters in Foam Cells Derived from RAW264.7 by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish an HPLC method for the determination of levels of intracellular cholesterol and cholesterol esters in foam cells derived from RAW264.7 murine macrophages so as to obtain a precise measure of foam cells and provide a technical platform for the study of the intracellular cholesterol content change.METHODS:HPLC method was applied to determine the content of cholesterol esters in the foam cells treated with OX-LDL and different concentration of pravastatin.RESULTS:OX-LDL could significantly enhance cellular intake of lipids(P