Extraction of Total DNA from Medicinal Lilii's Squama Leaves / 中国药房

ABSTRACT

OBJECTIVE:To improve and optimize the extraction process of DNA from medicinal lily. METHODS: A new feasible DNA extraction method was available by modifying and optimizing the traditional CTAB extraction method. RESULTS:The OD260/OD280 of the total DNA extracted by the modified CTAB method ranged from 1.6 to 2.0 with clear electrophoresis strip, and its content and purity were all up to the standards for RAPD analysis.CONCLUSION: A simple and feasible extraction process of DNA from medicinal Lily was found out and the content and purity of the DNA extracted in this method can meet the requirements for gene engineering.