Determination of Chloramphenicol and Dapsone in Compound Chloramphenicol Cream by HPLC / 中国药房
China Pharmacy
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-530159
ABSTRACT
OBJECTIVE:
To establish the HPLC method for the determination of chloramphenicol and dapsone in compound chloramphenicol cream.METHODS:
The analytical column was Hypersil ODS and the mobile phase consisted of 0.1%sodium heptanesulfonate solution (a mixture of 0.1% sodium heptanesulfonate 500 mL and dimethylformamide 5 mL plus acetic acid glacial 0.5 mL)-acetonitrile (75∶25) at a flow rate of 1.0 mL?min-1. The detection wavelength was 272 nm and the sample size was 20 ?L.RESULTS:
The linear ranges of chloramphenicol and dapsone were 25.59~153.54 ?g?mL-1 (r=0.999 9) and 12.57~75.42 ?g?mL-1 (r=0.999 9), respectively. The average recovery rates were 100.62% (RSD=0.92%) and 100.93% (RSD=1.22%), respectively.CONCLUSION:
The method is simple and accurate and can be used for the quality control of compound chloramphenicol cream.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2001
Type:
Article
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