Effects of BCG-PSN on the Expression of Interferon ? in Mice / 中国药房

ABSTRACT

OBJECTIVE: To detect the ability of polysaccharide nucleic acid fraction of bacillus calmette guerin(BCG-PSN) in inducing mIFN? in mouse. METHODS: The Balb/c mice were treated with saline, BCG-PSN and lipopolysaccharide(LPS) respectively, and mIFN? cDNA was amplified by RT-PCR. The ability of BCG-PSN in inducing mIFN? in mouse was identified through RT-PCR product with ?-actin as control. RESULTS: In normal sodium group, only ?-actin but not the mIFN? cDNA was detected, but in groups treated with BCG-PSN or LPS, mIFN? and ?-actin cDNA were all detected in RT-PCR amplification products. As compared with LPS group, there was stronger expression of mIFN? in BCG-PSN group (P