Effects of Yichan decoction(抑颤汤) on dopamine receptors and neurotensin in cerebral tissue of rats with Parkinson's disease / 中国中西医结合急救杂志

ABSTRACT

Objective To explore the mechanism of Yichan decoction(抑颤汤) in treatment of Parkinson′s disease(PD).Methods A PD rat model was made by direct injection of 6-hydroxydopamine(6-OHDA) into the brain to destruct the compact part of substantia nigra(SNC).One hundred Sprague-Dawley(SD) rats were randomly divided into a normal control group,a PD model group and a Yichan decoction treatment group and they were treated for 8 weeks.The rats′ behavior pattern changes were observed before treatment and from 5th weeks after treatment.The changes in the dissociation constant(Kd) and maximum number of binding sites(Bmax) of dopamine(DA) receptors,and neurotensin-immunoreactive(NT-IR) in substantia nigra after treatment were determined by using the technique of radioimmunity.Results Yichan decoction could markedly improve the rotation of PD model rats and decrease the average number of rotation from(349.56?62.28) circle/40 minutes to(61.63?17.93) circle/40 minutes(P