Determination of Vit B_1 and Vit B_2 in Duoweitie Oral Liquid by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To determine the contents of vitamin B1 and vitamin B2 in duoweitie oral liquid by HPLC.METHODS:Samples were separated on C18,the mobile phase consisted of ion pair solution(0.24% of triethylamine solution containing 1.5 mmol?L-1 of sodium pentanesulfonate and 3.4 mmol?L-1 of sodium heptanesulfonate with pH adjusted to 3.0 by glacial acetic acid)-methanol(80∶20) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 254 nm and the sample size was 10 ?L.RESULTS:The linear ranges of vitamin B1 and vitamin B2 were 4.81～24.6(r=0.999 1) and 6.22～31.7 ?g?mL-1(r=0.999 8),respectively,and the average recovery rates were 103.3%(RSD=0.5%) and 99.5%(RSD=0.7%),respectively.CONCLUSION:This method is simple,accurate,sensitive and reliable,and suitable for the determination of vitamin B1 and vitamin B2 in duoweitie oral liquid.