Expression of BRMSI in different metastasic breast cancer cells and its relation to HDAC activity / 中国普通外科杂志
Chinese Journal of General Surgery
; (12)1994.
Article
in Zh
| WPRIM
| ID: wpr-530491
Responsible library:
WPRO
ABSTRACT
Objective To investigate the expression of BRMS1 in different metastatic breast cancer cells and its relation to HDAC activity.Methods The gene expression of BRMS1 in 3 types breast cancer cell lines(non-metastasis MCF-7(A cell line),low metastasis MDA-MB-453(B cell line)and high metastasis MDA-MB-231(C cell line)) was determied by RT-PCR technique;the protein expression of BRMS1 was measured with Western blot technique and HDAC activity by enzyme linked immunosorbent assay.Results(1) Gene expressive ratio of BRMS1 was 3.1∶2.0∶1.0 in the A、B、C cell lines,respectively.The gene expression of BRMS1 in the C cell line decreased 210% compared to A cell line,and expression of BRMS1 was markedly reduced as the degree of metastasis increased(P
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WPRIM
Language:
Zh
Journal:
Chinese Journal of General Surgery
Year:
1994
Type:
Article