Effect of dominant negative Ⅰ?B? transfection on NF-?B pathway and cell motility in breast cancer / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1989.
Article
in Chinese
| WPRIM
| ID: wpr-530497
ABSTRACT
AIM:
To observe the effect on NF-?B pathway and cell motility in breast cancer cell lines after transfection of dominant negative I?B? plasmid.METHODS:
After stable transfection of mutant I?B? plasmid into highly metastatic breast cancer lines MDA-MB-231 and MDA-MB-435,we detected NF-?B binding activity by EMSA,cell growth ability by cell growth curve,colony forming test,and cell motility by millicell-PCF chamber.RESULTS:
Constitutive activities in MDA-MB-231 and MDA-MB-435 were observed.Stable transfection of a dominant negative Ⅰ?B? resulted in downregulation of NF-?B binding activity,thus inhibited cell mobility without significant effect on cell growth.CONCLUSION:
Cell migration ability is inhibited in highly invasive breast cancer cells by inhibition of NF-?B pathway in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Type:
Article
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