Effect of F10 gene on activities of transcriptional factor NF-?B and AP1 / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1989.
Article
in Chinese
| WPRIM
| ID: wpr-530503
ABSTRACT
AIM:
To investigate the influence of F10 on the activities of transcription factor NF-?B and AP1 in A549 cells.METHODS:
The luciferase report plasmids of NF-?B-Luc,AP1-Luc and F10 gene were introduced into A549 cells and the luciferase activity was detected.The DNA binding activities of AP1 and NF-?B in the cells were measured by the electrophoretic mobility shift assay(EMSA).RESULTS:
The luciferase activity in F10+ transfection group decreased 30% and increased 2-fold respectively 48 h after transfected with the luciferase report plasmid of NF-?B-Luc and AP1-Luc in A549 cells.The DNA binding activity decreased 49% and increased 23%,respectively.CONCLUSION:
F10 gene up-regulates the transcription activity of AP1 and down-regulates the NF-?B in A549 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Type:
Article
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