Effects of progesterone on the maturation and immunologic function of dendritic cells from human peripheral blood / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1989.
Article
in Chinese
| WPRIM
| ID: wpr-530628
ABSTRACT
AIM:
To study the effects of progesterone(P4) on the maturation and immunologic function of dendritic cells(DCs) from human peripheral blood.METHODS:
Cultured DCs were treated with P4 at doses of 10-7 mol/L and 10-6 mol/L.The morphologic changes were observed under the scanning electronic microscope.The immunophenotypes of DCs in control and treated groups were analyzed by flow cytometry.IL-10 and IL-12 production in culture supernatant was examined by ELISA assay.The capability of the stimulatory activity of the DCs on allogeneic T cells in mixed reaction was tested by incorporation of [3H]-TdR.RESULTS:
Compared with control group,cultured DCs in the presence of P4 displayed less dendritic pseudopod,expressed low levels of MHC-II,CD40,CD80 and CD86,and exhibited weakly activity in stimulating the proliferation of allogeneic T cells.Increase in IL-10 production and decrease in IL-12 production were observed.CONCLUSION:
P4 exerts negative effect on the maturation and immunologic function in dendritic cells from human peripheral blood.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Type:
Article
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