Determination of Protocatechuic Acid and Protocatechuic Aldehyde in Fuxuekang Granules by RP-HPLC / 中国药房
China Pharmacy
;
(12)2005.
Article
in Chinese
| WPRIM
| ID: wpr-530755
ABSTRACT
OBJECTIVE:
To establish a RP-HPLC method for the simultaneous determination of Protocatechuic acid and Protocatechuic aldehyde in Fuxuekang granules.METHODS:
The chromatography was carried on Achirom Bond-1 C18 column(250 mm?4.6 mm,5 ?m). The mobile phase was composed of methanol - water (20∶80,pH=2.80 adjusted by glacial acetic acid) at a flow rate of 1.0 mL?min-1. The detection wavelengths were programmed at 256 nm for Protocatechuic acid and at 280 nm for Protocatechuic aldehyde.RESULTS:
The linear ranges for Protocatechuic acid and Protocatechuic aldehyde were 5.49~73.22 ?g?mL-1(r=0.999 9) and 4.81~64.13 ?g?mL-1(r=0.999 9), respectively, and their average recoveries were 96.86%(RSD=2.52%,n=9) and 97.55%(RSD=3.82%,n=9) respectively.CONCLUSION:
The method is simple, sensitive, and reproducible, and it is applicable for the quality control of Fuxuekang granules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2005
Type:
Article
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