Fingerprint and Quality Evaluation of Apocynum Venetum Leaves / 中国药房

ABSTRACT

OBJECTIVE:To establish the HPLC fingerprints of Apocynum Venetum leaves, determine the content of Quercetin in the sample and evaluate the variety and quality of commercial Apocynum Venetum leaves. METHODS: Quercetin was separated on Zorbarx-C18(250 mm?4.6 mm, 5 ?m),and the mobile phase consisted of methanol and 0.4% H3PO4(gradient elution) at a flow rate of 0.9 mL?min-1 with the detective wavelength set at 360 nm. RESULTS: The precision of Quercetin was satisfactory(RSD=0.21%,n=6),and the solution of Apocynum Venetum leaves was reproducible and stable within 10 hours. CONCLUSION: This study serves as a scientific basis for the identification and quality evalution of Apocynum Venetum leaves.