Detection of Glibenclamide Mixed Illegally in Shenqi Hypoglycemic Granules / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for detection of glibenclamide mixed illegally in Shenqi hypoglycemic granules,a hypoglycemic traditional Chinese medicine preparation.METHODS:The constituents were identified by TLC,and the content of glibenclamide was determined by HPLC on Kromasil C18 column(250 mm?4.6 mm,5 ?m) with a ammonium dihydrogen phosphate(adjusted pH=3.15 with phospharic acid)-methanol(25∶75) as the mobile phase.The detection wave-length was set at 240 nm.RESULTS:The linear range of glibenclamide was 0.030 1～0.481 6 mg?mL-1(r=0.999 2) with an average recovery at 98.62%(RSD=0.54%,n=9).CONCLUSION:The method is sensitive and reliable,simple and feasible,and suitable for the detection of glibenclamide mixed illegally in hypoglycemic traditional Chinese medicine preparations.