Determination of Three Flavanones in the Effective Fraction of Flavone in Fructus Aurantii Immaturus by HPLC / 中国药房
China Pharmacy
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-531171
ABSTRACT
OBJECTIVE:
To establish an HPLC method for simultaneous determination of Naringin,Hesperidin and Neohesperidin in the effective fraction of flavone in Fructus Aurantii Immaturus.METHODS:
Hypersil C18 column(250 mm?4.6 mm,5 ?m) was used with the mobile phase consisted of acetonitrile -0.1% phosphoric acid solution(20∶80) at a flow rate of 1.0 mL?min-1,and the detection wavelength was set at 283 nm and the column temperature was maintained at 30 ℃.RESULTS:
The calibration curves of Naringin,Hesperidin and Neohesperidin were in good linearity over the ranges of 0.44~2.20 ?g(r=0.999 5),0.025 6~0.128 0 ?g(r=0.999 3),0.54 ~ 2.70 ?g(r=0.999 9),respectively.And the average recoveries for the three constituents were 99.41%,100.33% and 99.69%,respectively with RSD at 1.14%,1.47%,and 1.16%,respectively.CONCLUSION:
The method is simple,accurate and reliable,and can be used for the quality control of Fructus Aurantii Immaturus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2007
Type:
Article
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