Determination of Ephedrine Hydrochloride in Xiaoerkechuanling Granules by Two Different HPLC Methods:A Contrastive Analysis / 中国药房
China Pharmacy
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-531174
ABSTRACT
OBJECTIVE:
To determine the content of Ephedrine hydrochloride in Xiaoerkechuanling granules by two HPLC methods.METHODS:
The chromatographic conditions for the first HPLC method were as followsthe phenomenex C18 column(250 mm?4.6 mm,5 ?m) was used with methanol-water(1∶1) as mobile phase at a detection wavelength of 254 nm;the chromatographic conditions for the second HPLC method were as followsthe phenomenex C18 column(250 mm?4.6 mm,5 ?m)was used with acetonitrile-phosphoric acid solution(contain 0.02 mol?L-1 potassium dihydrogen phosphate and 0.01 mol?L-1 phosphoric acid)(5∶95) as mobile phase at a detection wavelength of 210 nm.RESULTS:
By HPLC method one,the linear range of Ephedrine hydrochloride was 0.08 ?g?mL-1~40 ?g?mL-1(r=0.999 9)with the average recovery at 101.72% and the RSD at 2.77%;by the second HPLC method,the linear range of Ephedrine hydrochloride was 0.4 ?g?mL-1~400 ?g?mL-1(r=1.000 0) with the average recovery at 99.60% and the RSD at 2.91%.CONCLUSION:
Both of the two HPLC methods are accurate,specific and reproducible,but by the first HPLC method,the extraction was more complete and more operable,and no organic solvent was used.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2007
Type:
Article
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