Involvement of JNK-initiated p53 accumulation and phosphorylation of p53 in pseudolaric acid B induced cell death
Experimental & Molecular Medicine
;
: 428-434, 2006.
Article
in English
| WPRIM
| ID: wpr-53147
ABSTRACT
A terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay was used to determine that apoptosis causes HeLa cell death induced by pseudolaric acid B. The c-Jun N-terminal kinase (JNK) inhibitor SP600125 decreased p53 protein expression during exposure to pseudolaric acid B. SP600125 decreased the phosphorylation of p53 during pseudolaric acid B exposure, indicating that JNK mediates phosphorylation of p53 during the response to pseudolaric acid B. SP600125 reversed pseudolaric acid B-induced down-regulation of phosphorylated extracellular signal-regulated protein kinase (ERK), and protein kinase C (PKC) was activated by pseudolaric acid B, whereas staurosporine, calphostin C, and H7 partly blocked this effect. These results indicate that p53 is partially regulated by JNK in pseudolaric acid B-induced HeLa cell death and that PKC participates in pseudolaric acid B-induced HeLa cell death.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Protein Kinase C
/
HeLa Cells
/
Tumor Suppressor Protein p53
/
Cell Death
/
JNK Mitogen-Activated Protein Kinases
/
Diterpenes
/
DNA Fragmentation
/
Anthracenes
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2006
Type:
Article
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