Determination of Capsaicin in Capsicum Annuum by RP-HPLC / 中国药房
China Pharmacy
;
(12)1991.
Article
in Chinese
| WPRIM
| ID: wpr-531846
ABSTRACT
OBJECTIVE:
To establish a RP-HPLC method for the determination of capsaicin in Capsicum annuum.METHODS:
The determination was performed on Zorbax Eclipse XDB-C18(150 mm?4.6 mm,5 ?m) column with column temperature set at 40 ℃.The mobile phase consisted of acetonitrile and 0.1% phosphate acid solution (45∶55,pH=2.6) with flow rate at 1.0 mL?min-1 and detective wavelength set at 281 nm.RESULTS:
The calibration curve of capsaicin was linear over the concentration range of 30~70 mg?L-1(r=0.999 9).The average recovery for capsaicin was 101.33 %(RSD=1.57%,n=9).CONCLUSION:
The method is simple,rapid and accurate,and suitable for the determination of capsaicin in Capsicum annuum.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
1991
Type:
Article
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