Expression of Stat3 signal transduction pathway in laryngeal carcinoma and its clinical significance / 中国耳鼻咽喉头颈外科

ABSTRACT

OBJECTIVE This study was conducted to investigate the expression of Stat3 and its target gene products including Cyclin D1 and Bcl-xl in human laryngeal cancerous tissue, and to explore the mechanism in tumorigenesis of larymgeal carcinoma, and to explore the correlation between the expression,clinical parameters as well as the pathological parameters of laryngeal carcinoma. METHODS The expression of Stat3, p-Stat3, Cyclin D1 and Bcl- xl in 50 cases of cancerous tissues, adjacent normal tissues was measured by Western blot analysis. The expression pattern of Stat3 and its activated form p-Stat3 was determined by immunohisto- chemical staining. The correlation of the expression of Stat3, p-Stat3, Cyclin D1 and Bcl-xl in laryngeal carcinoma with various clinicopathological characteristics was analyzed statistically. RESULTS The protein expression level of stat3, p-Stat3, Cyclin D1, Bcl-xl (A value)were 86.97?1.58, 56.97?3.93, 24.43?5.62, 33.78? 3.56 in laryngeal carcinoma and 36.10?0.52, 16.52?0.52, 5.94?1.75, 14.68?2.14 in adjacent normal respectively(P