Identification of Descurainia sophia and Semen Plantaginis by HPCE / 中国药房

ABSTRACT

OBJECTIVE:To identify Descurainia sophia and Semen Plantaginis by high performance capillary electrophoresis (HPCE). METHODS: HPCE was conducted with a fused silica capillary tube(60 cm?75 ?m) taking 30 mmol? L-1 sodium dihydrogen phosphate as running buffer (pH 8.2). The sample injection was performed by high pressure for 5 s at a separation voltage of 12 kV, an UV detection wavelength of 330 nm. RESULTS: The differences of HPCE electrophoregrams between Descurainia sophia and Semen Plantaginis were significant and easily to be differentiated. CONCLUSION: This method is simple, rapid and sensitive, and it can be applied to identify Descurainia sophia and Semen Plantaginis.