Determination of Benzhydramine Hydrochloride Content in Xiaoyan Zhentong Ointment by RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE: To determine the content of benzhydramine hydrochloride in Xiaoyan Zhentong ointment by RP-HPLC.METHODS: The determination was performed on Zorbax SB-CN column.Acetonitrile-water-triethylamine(51∶49∶0.5,adjusted to pH 6.5 with acetic acid glacial) was used as mobile phase at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 258 nm and the column temperature was set at 30 ℃.RESULTS: The linear range of benzhydramine hydrochloride was 0.1～4.1 ?g(r=0.999 9) with an average recovery rate at 98.4%(RSD=0.44%).CONCLUSION: The method is accurate,sensitive,reliable and applicable for the determination of the content of benzhydramine hydrochloride in Xiaoyan Zhentong ointment.