Determination of Seratrodast Concentration in Human Plasma by RP-HPLC / 中国药房
China Pharmacy
;
(12)2005.
Article
in Chinese
| WPRIM
| ID: wpr-532255
ABSTRACT
OBJECTIVE:
To establish a RP-HPLC method for the determination of seratrodast concentration in human plasma.METHODS:
The sample was determined on Lichrospher C18 column with the mobile phase consisted of 0.02 mol?L-1 potassium dihydrogen phosphate(containing 0.1% triethylamine,pH=5.0)-acetonitrile(70∶30)at a flow rate of 1.0 mL?min-1.The column temperature was 30℃ and the detective wavelength was set at 268 nm.RESULTS:
The linear range of seratrodast was 28~5 600 ng?mL-1(r=0.999 5)with the lowest detection concentration at 28 ng?mL-1.The recovery rates of seratrodast at low,middle and high concentrations were 102.9%,100.4% and 99.7%,respectively;and both intra-day RSD and inter-day RSD were all less than 6%.CONCLUSION:
The method developed in this study is applicable for the determination of plasma concentration and pharmacokinetic study of seratrodast.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2005
Type:
Article
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