Effects of Le Er Mai on apoptosis of hippocampus neuronal cells in anaphase of cerebral ischemic reperfusion injury in rats / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1989.
Article
in Chinese
| WPRIM
| ID: wpr-532274
ABSTRACT
AIM:
To investigate the effects and mechanism of Le Er Mai(LEM) on the apoptosis of hippocampus neuronal cells in the anaphase of cerebral ischemic reperfusion injury in rats.METHODS:
A rat model of middle cerebral artery occlusion reperfusion(MCAO) was produced with the intraluminal filament.During reperfusion for 30 d after 2 h of ischemia,the TUNEL staining methods were used to detect apoptosis of hippocampus neuronal cells,and immunohistochemical technique were employed to examine the protein expression of Fas,Bax,caspase-3 and caspase-9 in the hippocampial.The gene expressions of fas,bax,caspase-3 and caspase-9 in hippocampial were examined by RT-PCR.RESULTS:
After 2 h ischemia and 30 d reperfusion,compared with sham-operated group,TUNEL-positive staining cells and expression levels of Fas,Bax as well as caspase-3 and caspase-9 obviously increased,and the mRNA expressions of fas,bax,caspase-3 and caspase-9 in hippocampial markedly up-regulated in model group.Compared with model group,LEM at dose of 2.00 g/kg or 0.87 g/kg,and flunarizinum significantly reduced apoptosis and decreased the protein expressions of Fas,Bax,caspase-3 and caspase-9 in hippocampial,and down-regulated the mRNA expressions of fas,bax,caspase-3 and caspase-9(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Type:
Article
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