Determination of Three Active Constituents in Coptidis Decoction for Detoxification by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish an HPLC method for the determination of the contents of baicalin,berberine hydrochloride and jasminoidin in Coptidis decoction for detoxification.METHODS:The samples were separated on Shimazu Sim-pack VP-ODS C18(250 mm?4.6 mm,5 ?m)with mobile phase consisted of acetonitrile-(0.05% potassium dihydrogen phosphate+0.05% trolamine solution,with pH adjusted to 3.0 by phosphoric acid)in gradient elution under a detection wavelength of 254 nm.RESULTS:The linear ranges of jasminoidin,berberine hydrochloride and baicalin were 0.4～2.0 mg?mL-1(r=0.998 1),0.020～0.40 mg?mL-1(r=0.992 0)and 0.020～0.40 mg?mL-1(r=0.996 3),respectively,and their average recoveries were 99.8%,97.5% and 98.1%,respectively,with RSD at 1.8%,2.5% and 1.5%,respectively(n=6 for all the three).CONCLUSION:The established method is specific,sensitive,simple and applicable for the quality control of Coptidis decoction for detoxification.