Determination of Four Flavonoids in Herba Euphorbiae Humifusae by RP-HPLC / 中国药房
China Pharmacy
;
(12)2005.
Article
in Chinese
| WPRIM
| ID: wpr-532469
ABSTRACT
OBJECTIVE:
To establish a RP-HPLC method for simultaneous determination of 4 flavonoids in Herba Euphorbiae Humifusae.METHODS:
The samples were separated on Hypersil BDS C18 column(150 mm?4.6 mm,5 ?m)with CH3OH-0.3%H3PO4 as mobile phase(gradient elution)at a flow rate of 1.0 mL?min-1.The column temperature was maintained at 30 ℃ and the detective wavelength was set at 360 nm.RESULTS:
The linear ranges of 4 flavonoids including rutin,quercetin,luteolin and kaempferol were 0.127 2~2.034 4 ?g(r=0.999 9),0.089 1~1.425 6 ?g(r=0.999 8),0.076 6~1.226 4 ?g(r=0.999 7),0.113 0~1.808 0 ?g(r=0.999 8)respectively,and their average recoveries were 97.6%,99.4%,98.1% and 101.9%,respectively with RSD at 0.94%,0.73%,0.99% and 1.34%,respectively(all n=9).CONCLUSION:
This method was proved to be simple,accurate,reproducible and applicable for the quality control of Herba Euphorbiae Humifusae.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2005
Type:
Article
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