In vitro killing effect of adenovirus-mediated herpes simplex virus thymidine kinase gene under regulation of hypoxic response element on hepatoma cell line HepG2 / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-532698
ABSTRACT
AIM:
To investigate the in vitro killing effect of adenovirus-mediated herpes simplex virus thymidine kinase gene(HSV-TK) driven by hypoxic response element(HRE) on hepatoma cell line HepG2.METHODS:
Recombinant adenoviral vector Ad-HRE-TK was constructed with HSV-TK under the control of HRE using AdEasy system.Then Ad-HRE-TK was transfected into hepatoma cell line HepG2 and the cells were cultured under normoxic or hypoxic conditions.After treated with GCV for 3 d,the sensitivity to GCV of HepG2 was measured by MTT method.RESULTS:
Over 95% HepG2 cells infected with Ad-HRE-TK cultured under hypoxic condition were killed when the MOI was 100 and the concentration of GCV was 50 mg/L.On the contrary,no killing effect of GCV was observed in cells cultured under normoxic condition.CONCLUSION:
HRE promotes the expression of HSV-TK specifically under hypoxic condition and induces the specific killing effect of GCV.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2000
Type:
Article
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