Effect of fractalkine on the expression and secretion of MMP-2 in human monocytes / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-532703
ABSTRACT
AIM:
To study the effect of fractalkine(CX3CL1,Fkn) on the expression and secretion of matrix metalloproteinase-2(MMP-2) in cultured human monocyte line U937 cells.METHODS:
The cultured U937 cells were incubated with recombinant human Fkn,the supernatant of human renal mesangial cells(HRMC) and Fkn neutralizing antibodies for 24 h.The mRNA expression of MMP-2 was analyzed by RT-PCR.The production of MMP-2 in the supernatant was analyzed by gelatin zymography.RESULTS:
The level of MMP-2 mRNA and protein in the cells incubated with recombinant human Fkn decreased compared to control group.Similarly,the level of MMP-2 mRNA in the cells incubated with the supernatant of HRMC reduced compared to control group.However,the level of MMP-2 mRNA and protein in the cells incubated with the supernatant of HRMC adding Fkn neutralizing antibodies increased compared to that incubated with the supernatant of HRMC.CONCLUSION:
Fkn inhibits the expression and secretion of MMP-2 in cultured U937 cells.HRMC might mediate the expression and secretion of MMP-2 in U937 cells through Fkn.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2000
Type:
Article
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