Protective effect of bivalent anti-snake venom IgY administered intragastrically on mice with cobra or viper envenomation / 中国病理生理杂志

ABSTRACT

AIM:The different effect of bivalent immunoglobulin Yolk(IgY) was evaluated against snake venom between intragastric administration and intraperitoneal injection in mice with cobra or viper envenomation.METHODS:The venom of naja and viper was injected alternately into the leghorn hen.Bivalent anti-snake venom IgY was extracted by water dilution.The concentration of bivalent IgY in plasma was observed in indirect ELISA assay after bivalent anti-snake venom IgY taken orally.The gastric emptying function test was used for determining optimization time after gastric administration of IgY.The protective effect of bivalent anti-snake venom IgY was compared between intragastric administration and intraperitoneal injection in mice with cobra or viper envenomation.RESULTS:Bivalent anti-snake venom IgY was extracted from eggs laid in 28-42 d after the first immunization.The titers of Bivalent IgY against cobra and viper venom were 112 800 and 16 400.At the time of 2.5-3.5 h after bivalent anti-snake venom IgY was taken orally in three concentrations(75 mg,150 mg,300 mg?0.5 mL-1?20 g-1 BW),the gastric evacuation rate of mice was above 68.9%,with the plasma concentration of bivalent IgY in peak.The survival time of mice envenomation with snake venom was extremely prolonged(P