Simultaneous Content Determination of Paracetamol and Dioxopromethazine Hydrochloride in Compound Paracetamol and Zinc Gluconat Tablets by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a HPLC method for simultaneous determination of paracetamol and dioxopromethazine hydrochloride in compound paracetamol and zinc gluconat tablets. METHODS: The determination was performed on Sinochrom ODS-BP column with the mobile phase consisting of 0.04 mol?L-1 KH2PO4 (pH=2.60,0.40% triethylamine)-acetonitril (75∶25) at a flow rate of 1.0 mL?min-1;the detection wavelength was set at 264 nm;the column was maintained at room temperature and the sample size was 20 ?L. RESULTS: The calibration curve was linear over the range of 2.818? 10-3～3.605?10-1 mmol?L-1 for paracetamol and 1.118?10-4～1.431?10-2 mmol?L-1 for dioxopromethazine hydrochloride. The recovery rates of both were greater than 99.2%. The intra-day RSDs of both were less than 1.82% and the between-day RSDs of both were less than 1.90%. CONCLUSION: The method is rapid,simple and accurate,and it is applicable for the quality control of the tablets.