Influence of Jiaomu oil A2 on eosinophil manifold and CD34~+ with marrow granule system mobilization in bronchia asthma mice / 中国病理生理杂志

ABSTRACT

AIM: To observe the influence of Jiaomu oil A2 on eosinophil manifold and CD34+ with marrow granule system mobilization in bronchial asthma mice.METHODS: The asthmatic mouse model was established by sensitization and challenge of the animals with 20% Al(OH)3+10% ovalbumen(OVA).After the mice were excitated for 10 d and giving medical therapy at the same time,the mice were executed,the bronchial-alveolar lavage inflammatory cells and the hemocytopoiesis cells were examined using Wrish-Giemsa staining.The expressions of interleukin-5(IL-5) and eotaxin(EON) in lung tissue and marrow were examined by in situ hybridization.The expression of IL-5 and EON albumen in lung tissue and marrow were detected by immunohistochemistry.The inflammatory cell infiltration and CD34+ cells in lung tissue were also observed by HE and immunofluorescence staining.RESULTS: Both Jiaomu oil A2 and prednisone significantly attenuated the pathological process degree of bronchial inflammatory reaction such as tissue swelling,reconstruction,hyperplasia,and epithelial cell shedding,and inhibited eosinophil count and infiltration caused by stimulation of allergic effect.Moreover,the two drugs markedly lessen the eosinophil density in tracheal surrounding tissue and marrow in asthma mice and depressed the differentiation of marrow myelocyte to eosinophil.Finally,the apparent decrement of CD34+IL-5,CD34+IL-5R,CD34+CCR-3 cells in bronchial tissue and marrow showed some relationship with the downregulation of IL-5,IL-4,GM-CSF in lung tissue.CONCLUSION: The effect of Jiaomu oil on airway inflammation in asthma mice is associated with inhibiting the mobilization of eosinophil and marrow granule system.